The members of staff, who have not yet been named, were in a blue Subaru Impreza when it collided with a Ford Transit van on the A320 in Ottershaw near Woking, Surrey.
They were based at the nearby McLaren Production Centre, next to where the company's F1 cars are built, and worked for the firm's sports car division.
Mike Flewitt, chief executive officer of McLaren Automotive, said: "We have been informed that we have sadly lost two hard working and extremely well-liked employees.
"They will be sorely missed by their friends and colleagues and our heartfelt condolences go out to their families."
The crash, which happened between the McLaren and Otter roundabouts, closed the A320 Guildford Road for several hours.
Surrey Police appealed for witnesses to the crash to contact them on 101.